Indian Journal of Veterinary Surgery
Year : 2006, Volume : 27, Issue : 1
Print ISSN : 0254-4105.
Title of paper: Efficacy of fresh and preserved foetal cortical allografts in the repair of bone defects in dogs
Authors:
Rajesh K.U.1, M.V.Sc. scholar, Sharma V.K.2†, Professor and Head, Sharma Amit1, M.V.Sc. scholar
G.B. Pant University of Agriculture and Technology, Pantnagar – 263 145 (Uttaranchal)
1Department of Surgery and Radiology, College of Veterinary and Animal Sciences, Pantnagar
2Department of Surgery and Radiology, College of Veterinary and Animal Sciences, Pantnagar
†Corresponding author Foetal cortical allografts
The osteoinductive potential of fresh and preserved foetal cortical allografts was studied in 27 dogs divided randomly in 3 equal groups A, B and C. Dogs were having an osseous defect of approximately 1 cm x 1 cm in the mid-diaphysis of radius. In group A dogs, the defects were left unrepaired (control), while the defects in groups B and C were filled with fresh and preserved foetal cortical allografts, respectively. Foetal allografts were collected from dead foetuses obtained by hysterectomy performed on dead pregnant bitches within 30 min after their death due to automobile accident or fall from height.
The foetal long bone diaphyses were collected and sectioned to the desired dimensions to fit in the radial defects. Some parts of the foetal bony pieces were preserved in dextrose normal saline (5%) for 25 days under refrigeration at 4°C temperature and used as preserved graft in groups C animals and remaining part of bony pieces were used as fresh grafts in group B animals to repair radial defect.
The osteoinducing potential of the grafts was evaluated on the basis of clinical, histological, hisochemical and tetracycline labeling studies. Histological and histochemical studies showed mild deposition of cartilaginous tissue close to the defect and moderate mucopolysaccharide activity in control group, while in group B extensive deposition of collagen and mucopolysaccharides, and in group C moderately localized deposition of collagen and mucopolysaccharide was observed at the host-graft interface at 60th day.
It was concluded that both fresh and preserved cortical bone allografts possessed osteoinductive and osteoconductive properties. However, the fresh grafts proved to be superior than the preserved grafts.
