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[1] Methods: Simple but powerful super-resolution microscopy
DOI: 10.1038/nmeth.1812
A simple yet powerful method for performing super-resolution microscopy on living cells expressing fluorescent proteins is reported in Nature Methods. Using this methodology, cell biologists should be able to readily increase the resolution of their current live-cell imaging experiments using fluorescent proteins by fourfold and observe previously hidden details of the underlying biology.
Though there are a plethora of super-resolution microscopy methods, they do not provide the accessibility and utility needed by many biologists.
Susan Cox and colleagues describe a method for analyzing images generated during high-speed fluorescence imaging of fluorescently tagged proteins in living cells under common arc lamp illumination. The method allows them to generate super-resolution images with high temporal and spatial resolution over extended periods and a generous field of view. Existing methods have been unable to provide the same level of performance while maintaining the conditions amenable to live cell imaging that are so crucial to cell biologists.
Author contact:
Susan Cox (King's College London, UK)
Tel: +44 207 848 6519; E-mail: [email protected]
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