Title of Paper: APPLYING PROTEOMICS TO DRUG DISCOVERY: ISOLATION OF CO-II AS AN ACTIVE CONSTITUENT AGAINST BREAST CANCER CELLS FROM MALAYSIAN PLANT CFPR-110
Mohd Ilham A., Asiah O., Nurhanan M.Y., Li Yen C., Siti Syarifah M.M., Norhayati I., Lili Sahira H. and Jaya V.
Forest Research Institute Malaysia, Kepong, 52109 Selangor, Malaysia
Tel: 03-62797652
Fax: 03-62804623
Email: [email protected]
Until recently, the growth of proteomics has been hindered by a lack of available tools and poor integration. Through the combination of new analytical tools and information technologies, researchers are now able to use a proteomic approach to quickly identify changes in proteins associated with disease.
Centre for Proteomic Research (CfPR) of FRIM is an advanced proteomic research and development centre focusing on drug discovery from biodiversity resources. The centre is equipped with state-of-the-art instruments generally to address two technical issues: the separation of proteins, and their identification and characterization.
Under the anti-cancer screening program carried out at the centre, 31 plant species were screened for anti-breast cancer activity. An active plant coded as CfPR-110 was found to have notable anti-proliferative activity against breast cancer cells, MCF-7 and T47D.
Bioassay guided fractionation of the ethyl acetate layer of CfPR-110 yielded active constituent, CO-II. This study highlights the integrated approach of proteomics, which include the use of two-dimensional gel electrophoresis (2-DE) and mass spectrometry to facilitate the detection and identification of proteins from the treated and non-treated cell lysates.
This paper also discusses the separation of ethyl acetate extract of CfPR-110 into several individual chemical components, including CO-II using a patented combination of High Performance Chromatography (HPLC) and Solid Phase Extraction (SPE) equipment i.e. a Sepbox 2D-100.
This research was presented at the CFFPR 2007 Meeting.
For more information on FRIM and the CFFPR Meeting, please contact Dr Mohd. Nor Mohd. Yusoff, Senior Director, Forest Products Division, Forest Research Institute Malaysia (FRIM). Email: [email protected]
