More efficient bioethanol production might be possible using persimmon tannin to help yeast thrive

Naturally derived antioxidants improve growth of yeast strain in presence of ethanol

Persimmon tannin powder was used to create the supernatant that showed some beneficial antioxidative properties that help yeast grow.

While ethanol in alcoholic beverages impairs drinkers’ motor functions, it is that same substance that can power motor vehicles in a cleaner, more sustainable manner. What is necessary for the production of ethanol is yeast, but ethanol is among the environmental factors that add stress to yeasts, hindering their growth. To promote efficient bioethanol production, scientists have been searching for substances that can help yeasts better withstand ethanol, but few effective ones have been found.

An Osaka Metropolitan University research team, including graduate student Ilhamzah and Professor Ken-ichi Fujita of the Graduate School of Science and Professor Akira Ogita of the Research Center for Urban Health and Sports, has found that tannin from persimmons improves the growth of the yeast strain Saccharomyces cerevisiae in the presence of ethanol.

“In this study, yeast cultures grown in a medium containing ethanol and persimmon tannin showed an 8.9-fold increase in cell number compared to cultures grown in an ethanol medium without persimmon tannin,” stated Professor Fujita.

The researchers explored persimmon tannin because it is known for its antioxidative properties.

“Persimmon tannin reduced ethanol-induced oxidative stress,” Fujita added. “However, persimmon tannin did not prevent ethanol-induced cell membrane damage. This indicates the potential of persimmon tannin as a protective agent to enhance the yeast’s tolerance to ethanol stress by limiting oxidative damage, rather than limiting damage to the yeast’s cell membranes.”

The findings were published inthe Journal of the Science of Food and Agriculture.

Conflict of Interest

The authors declare no conflict of interest.


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Published: 16 May 2024

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Rina Matsuki

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Osaka 558-8585 JAPAN

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Journal: Journal of the Science of Food and Agriculture
Title: Persimmon tannin promotes the growth of Saccharomyces cerevisiae under ethanol stress
DOI: 10.1002/jsfa.13439
Author(s): Ilhamzah, Yuka Tsukuda, Yoshihiro Yamaguchi, Akira Ogita, Ken-ichi Fujita
Publication date: 6 March 2024

Funding information:

This study was partially funded by the Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research (C) 19K05799 and 21K07029.