Offspring of mice fed imbalanced diets shown to be neurologically ‘programmed’ for obesity

Pregnant mice fed diets high in omega-6 fats and low in omega-3 fats are shown in a new study to produce offspring whose brains had a higher level of dopamine-producing neurons—the neurological reward system. These mice went on to chase hyper-caloric diets, suggesting that the fats in a pregnant mother's diet may control the eating habits of her children, and potentially offering a new obesity-prevention strategy.

Image title: Newly generated brain cells that release dopamine, which drive hedonic eating in adulthood

Image caption: The magenta staining shows brain cells that release dopamine. The green staining shows newly generated cells. The blue staining shows all cells.

Pregnant mice fed a diet high in omega-6 fats and low in omega-3 fats produce offspring that go on to exhibit “hedonic”—pleasurable but excessive—levels of consumption of hyper-caloric diets, according to researchers at Hiroshima University.

Omega-6 fats are found in grapeseed oil, corn oil and sesame oil, and are a staple of several salad dressings in world cuisine. Omega-3 fats are found in fish, perilla oil, and linseed oil. A diet balanced with these fats is considered essential for healthy brain growth.

The researchers also found that the offspring exhibit increased in utero growth of dopamine-producing neurons in the midbrain—the neurological reward system. They believe that exposure to this high omega-6/low omega-3 diet increases growth in these neurons in the fetus’s brain during a specific period during pregnancy, driving dopamine release in the offspring's brain, and thus primes the offspring for hedonic consumption of sugar- or fat-rich diets over the course of their life.

The findings were published in the peer-reviewed journal Communications Biology, on August 28.

Meanwhile, mice whose mothers had not consumed the imbalanced omega-6/omega-3 diet did not exhibit as much overeating behavior, even when tempted by the presence of such food.

Since the 1960s, the Western diet has experienced a significant uptick in the presence of polyunsaturated omega-6 fats, and in ratios to polyunsaturated omega-3 fats that historically humans had never experienced before.

The ratio between these two types of fats is important because biochemically they compete with each other for incorporation into cell membranes, and an omega-6/omega-3 imbalance in the membranes of red blood cells is correlated with weight gain. An earlier study on mice had found that consumption of an imbalanced omega-6/omega-3 diet by the pregnant mother replicates this imbalance in the offspring's brain and even impairs brain development.

The Hiroshima researchers also found that a dopamine-inhibiting drug eliminates the hedonic consumption of the offspring, further supporting the notion that the dopamine signaling plays a critical role in driving this behavior.

“This suggests that adult mice gorging themselves on hyper-caloric diets were in effect neurologically programmed to do so by their mother’s own consumption patterns,” said Nobuyuki Sakayori, paper author and assistant professor from the Graduate School of Biomedical and Health Sciences at Hiroshima University.

The scientists were keen to stress that the ratio of omega-6 to omega-3 fat in the mouse diet is much higher than that experienced by most humans, and that their work lays the foundation for further, epidemiological studies on humans to see if the pattern holds for us.

But if it does, this could provide a new strategy for preventing obesity in children by managing the type of fats that pregnant mothers consume, akin to how mothers today generally avoid consumption of alcohol.

“This could work much better than existing anti-obesity campaigns or food taxes,” Sakayori continued, “because instead of fighting against the brain’s reward system, such a strategy focuses right from the start on the development of that system.”


Since its foundation in 1949, Hiroshima University has striven to become one of the most prominent and comprehensive universities in Japan for the promotion and development of scholarship and education. Consisting of 12 schools for undergraduate level and 4 graduate schools, ranging from natural sciences to humanities and social sciences, the university has grown into one of the most distinguished comprehensive research universities in Japan. English website:

Published: 04 Sep 2020


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Sakayori, N., Katakura, M., Hamazaki, K. et al. Maternal dietary imbalance between omega-6 and omega-3 fatty acids triggers the offspring’s overeating in mice. Commun Biol 3, 473 (2020).

Funding information:

This work was supported by JSPS KAKENHI Grant Numbers JP16H06276, JP17H06059, JP17J10395, JP19H05023, and JP19K20184 (to N.S.), the Grant for Young Scientists from the Japan Society of Nutrition and Food Science (to N.S.), and the Otsuka Award from the Japan Society for Lipid Nutrition (to N.S.).